UV-2600/2700

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    Hardware Specifications

    Item UV-2600 UV-2700
    Photometric system Double-beam optics
    Photometric system Czerny-Turner mounting Single monochromator Lo-Ray-Ligh grade blazed holographic grating Czerny-Turner mounting Double monochromator Lo-Ray-Ligh grade blazed holographic grating
    Detector R-928  Photomultiplier
    Light source 50 W halogen lamp, deuterium lamp, light source auto position adjustment built in
    Setting wavelength range 185–1400 nm
    Measurement wavelength range 185–900 nm
    220 nm to 1400 nm when the ISR-2600Plus Integrating Sphere Attachment is used. The ISR-2600Plus Integrating Sphere Attachment cannot be used
    Wavelength accuracy ± 0.1 nm (656.1 nm D2), ± 0.3 nm (all range)
    Wavelength repeatability ± 0.05 nm
    Wavelength scanning speed Wavelength slew rate: about 14000 nm/min Wavelength scan rate: about 4000 to 0.5 nm/min
    Wavelength setting At 1 nm units for scan start and scan end wavelengths, and 0.1 nm units for other wavelengths
    Lamp interchange wavelength Auto switching synchronized with wavelength; switching range selectable between 290 and 370 nm (0.1 nm units)
    Spectral bandwidth 0.1/0.2/0.5/1/2/5 nm L2/L5 (low stray-light mode)
    Resolution 0.1 nm
    Stray light Max. 0.005 % (220 nm, NaI) Max. 0.005 % (340, 370 nm, NaNO2) Max. 1 % (198 nm, KCl) Max. 0.00005 % (220 nm, NaI) Max. 0.00002 % (340, 370 nm, NaNO2) Max. 1 % (198 nm, KCl)
    Photometric modes Absorbance (Abs.), transmittance (%), reflectance (%), energy €
    Photometric range Absorbance: −5 to 5 Abs Transmittance, reflectance: 0 to 100000 % Absorbance: −8.5 to 8.5 Abs Transmittance, reflectance: 0 to 100000 %
    Photometric accuracy ± 0.002 Abs (0.5 Abs) ± 0.003 Abs (1 Abs) ± 0.006 Abs (2 Abs) ± 0.3 %T Measured using NIST930D/NIST1930 or equivalent filter
    Photometric repeatability ± 0.001 Abs (0.5 Abs) ± 0.001 Abs (1 Abs) ± 0.003 Abs (2 Abs) ± 0.1 %T
    Noise level 0.00003 Abs RMS (500 nm) 0.00005 Abs RMS (500 nm)
    Baseline flatness  ± 0.0003 Abs (200–860 nm), 1 hour after light source is turned ON ± 0.0004 Abs (200–860 nm), 1 hour after light source is turned ON
    Baseline stability  Within 0.0002 Abs/h (700 nm), 1 hour after light source is turned ON Within 0.0003 Abs/h (700 nm), 1 hour after light source is turned ON
    Sample compartment Internal dimensions: W150 × D260 × H140 (mm) Distance between light beams: 100 mm Maximum optical path length of cell: 100 mm
    Dimensions W450 x D600 x H250(mm)
    Weight 23 kg
    Operating temperature 15°C to 35°C
    Operating humidity 30 % to 80 % (no condensation, less than 70 % above 30 °C)
    Power requirements 100 to 240 VAC, 50/60 Hz
    Power consumption 170 VA

    Software Specifications 

    UVProbe Software

    Operating System Windows 7 Professional (64/32 bits)
    Data Acquisition Modes Spectrum, Photometric (quantitation), and Kinetics (time course)
    General
    • Multitasking (possible to execute data processing while measurement is being performed)
    • Customizable measurement screen layout (wavelengths, data display font and font size, colors, displayed number of digits)
    • GLP/GMP compliant (security, history)
    • Real-time concentration display
    Spectrum Mode
    • Comparison/interactive processing of multiple spectra (Note 2)
    • Storage of all processed data with original data set including a history of all manipulations
    • Spectrum enlargement/shrinking and auto scale
    • Automatic interlink with spectral data processing
    • Annotation on spectrum screen
    Data Processing in Spectrum Mode
    • Normalization, Point Pick, peak/valley detection, area calculation
    • 1st – 4th derivatives, smoothing, reciprocal, square root, natural log, Abs, %T conversion, exponential conversion, and Kubelka-Munk conversion
    • Ensemble averaging, interpolation, four arithmetic operations (between spectra, between spectra and factors)
    Photometric (Quantitation) Mode
    • Single wavelength, multi-wavelength (includes 1, 2, or 3 wavelengths), spectrum quantitation (peak, maximum, minimum, area, etc. for specified wavelength ranges)
    • K-factor, single-point, multi-point calibration curves (1st, 2nd, 3rd order function fitting, pass-through-zero specification)
    • Photometric processing with user-defined functions (+, − , × , ÷ , Log, Exp, etc. functions, including factors)
    • Weight correction, dilution factor correction, and other corrections using factors
    • Averaging of repeat measurement data
    • Simultaneous display of standard sample table, unknown sample table, and calibration curves
    • Display of Pass/Fail indications
    Kinetics (Time Course) Mode
    • Comparison/interactive processing of multiple time-course data (Note 2)
    • Single- or double-wavelength measurement (difference or ratio)
    • Simultaneous display of time-course data, enzyme table, and graphs
    • Enzyme kinetics calculation (for single- or multi-cell)
    • Michaelis-Menten calculations and graph creation (Michaelis-Menten, Lineweaver-Burk, Hanes, Woolf, Eadie-Hofstee), Dixon plot, Hill plot
    • Integrated management of sample information including original data, sample weight, and dilution factors, etc.
    • Event recording such as addition of reagents during measurement
    • Time-course spectrum data processing (same as in spectrum data processing)
    Report Generator
    • Preview and print functions for customized formats
    • Layout and editing of templates
    • Quick printing using report templates
    • Auto-printing (spectrum mode)
    • Multi-page printout
    • Insertion of date, time, text, and drawing objects including lines, circles and rectangles
    • Insertion of spectral/quantitative data, method, and history
    • Insertion of headers and footers
    • Specification of graph line thickness (for each module), font style, and size

     

    UV Validation Softwar

    Inspection Items  Initialization results log • Wavelength accuracy • Wavelength repeatability • Photometric accuracy • Photometric repeatability • Resolution • Stray light • Baseline flatness • Noise level • Drift (baseline stability)
    Features • Select the items to implement during inspection to confirm the approximate time required for full inspection.
    • The system is capable of both wavelength repeatability checks and wavelength accuracy checks using a wavelength calibration filter. If the optional mercury lamp unit is used, wavelength accuracy can be inspected using the low-pressure mercury lamp's bright line.
    • The software is capable of resolution inspections using methods defined in the EP (European Pharmacopoeia) and USP (United States Pharmacopoeia) as well as the method for checking the bright line spectral bandwidth.